Purification of post-transcriptionally modified tRNAs for enhanced cell-free translation systems
Evan M. Kalb, Jose L Alejo, Leticia Dias-Fields, Isaac J. Knudson, Joshua A. Davisson, E. Maldonado, Kanokporn Chattrakun, Shangsi Lin, Jung Yeon Lee, Tianchen He, Alanna Schepartz, Shenglong Zhang, Scott C. Blanchard, Aaron E. Engelhart, Katarzyna P. Adamala
This paper develops a method to purify post-transcriptionally modified tRNAs from E. coli, enhancing translation yields and fidelity in cell-free systems.
Nucleic Acids Research · Feb 24, 2026Peer-reviewedBiochemistry