Alanna Schepartz

The paper demonstrates a method to multiplex FLIM using small molecule probes modified with bioorthogonal chemistry to achieve distinct fluorescent lifetimes without transfection.

This study improves cross-presentation by using fusion-dependent mini-proteins to enhance antigen delivery to the dendritic cell cytosol, leading to better MHC-I presentation and T-cell activation.

This paper demonstrates a prebiotic, nonenzymatic aminoacylation process that couples RNA and protein chirality, favoring opposite L- and D- configurations.

This study shows that an A2062U mutation in 23S rRNA improves ribosome activity for translating sequences with challenging monomers like proline and β2-hydroxy acids.

This paper develops a method to purify post-transcriptionally modified tRNAs from E. coli, enhancing translation yields and fidelity in cell-free systems.

This paper presents a method for high-yield, low magnesium flexizyme reactions in a water-ice eutectic phase, improving product stability and reducing Mg(II) carryover.

The paper introduces a new assay called PARTI to measure monomer-specific acyl-tRNA levels in cells and applies it to study non-amino acid monomer incorporation into proteins.

This paper shows that altering the timing of endosomal escape with AV5.3 improves enzymatic activity of delivered cargo compared to ZF5.3.

This study compares electroporation and packaged RNP delivery for genome editing, finding that packaged delivery is significantly more efficient and faster.

The paper confirms a new archaeal genetic code (Code 34) where TAG codons encode pyrrolysine, showing sporadic distribution and lateral gene transfer.

This study investigates how β-amino acid isomers affect ternary complex stability and translation elongation by analyzing their impact on ribosome function and EF-Tu interactions.

This paper introduces a translation-independent method to engineer aminoacyl-tRNA synthetases that can incorporate noncanonical amino acids into proteins.

This paper identifies the requirements for efficient endosomal escape by ZF5.3, a mini-protein, showing pH-induced unfolding and lipid-BMP interaction are critical for its function.

The paper describes the design and characterization of mini-PTC 1.1, a minimal ribosomal PTC RNA optimized through community science and RNA structural analysis.

The paper demonstrates the synthesis of proteins with multiple β2-hydroxy acid backbones in vivo using an orthogonal aminoacyl-tRNA synthetase.

This paper introduces a method to synthesize proteins with internal β2-peptide linkages in cells by using a post-translational rearrangement after incorporating specific -hydroxy acid monomers.

The paper uses atomistic simulations to identify structural criteria for substrates that can be efficiently incorporated by the E. coli ribosome.

The paper develops a stapled peptide that inhibits p53-Mediator interaction, suppressing p53 transcriptional activity in cancer cells.

This paper reports PylRS enzymes that accept non-proteinogenic monomers for in vitro translation, enabling the synthesis of sequence-defined hetero-oligomers.

This paper demonstrates that the engineered organism Vmax X2 enables high-yield and high-fidelity incorporation of non-canonical amino acids into proteins.

The paper demonstrates a triply orthogonal system that reassigns three stop codons to encode three distinct noncanonical amino acids in proteins.