Evan M. Kalb

This paper demonstrates a prebiotic, nonenzymatic aminoacylation process that couples RNA and protein chirality, favoring opposite L- and D- configurations.

This paper develops a method to purify post-transcriptionally modified tRNAs from E. coli, enhancing translation yields and fidelity in cell-free systems.

This paper presents a method for high-yield, low magnesium flexizyme reactions in a water-ice eutectic phase, improving product stability and reducing Mg(II) carryover.

This study identifies suitable tRNA sources for cell-free protein synthesis by evaluating tRNA pools from different E. coli strains and V. natriegens, finding that A19 tRNAs are most effective for in vitro translation.