Lysis buffer selection guidance for mass spectrometry-based global proteomics including studies on the intersection of signal transduction and metabolism
Barbara Helm, Pauline Hansen, Li Lai, Luisa E. Schwarzmüller, Simone Menghai Clas, Annika Richter, Max Ruwolt, Fan Liu, Dario L. Frey, Lorenza A. D’Alessandro, Wolf‐Dieter Lehmann, Marcel Schilling, Dominic Helm, Dorothea Fiedler, Ursula Klingmüller
This study compares four lysis buffers for proteomics, finding SDS most reliable and advocating a dual-sample approach to maximize proteome coverage.
bioRxiv · Feb 19, 2024 · PreprintSystems Biology