Fan Liu

This study identifies structural elements and dynamics of the Mic60-Mic19 subcomplex crucial for MICOS assembly and crista junction formation.

The paper reports an extended structure of the human nuclear pore complex, revealing TREX-2 components as integral parts of the nuclear ring.

The paper maps the spatial and structural proteome architecture of HEK293 cells using subcellular fractionation and cross-linking mass spectrometry, identifying residue-level protein interactions and expanding the human cell interactome.

This study shows how Tau condensates age into elastic networks with inhomogeneous polymerization and seed formation, providing mechanistic insight into their transition to pathological aggregates.

The paper investigates the role of hypothalamic NPY/AgRP neurons in regulating age-related presynaptic remodeling through autophagy and non-cell autonomous mechanisms.

This study maps HSV-1 virion-wide protein interactions, revealing maturation-driven network remodeling and conserved organizational principles across herpesviruses.

The paper maps virus-host protein contact sites during influenza A virus infection, revealing new interactions and paraspeckle disassembly as a novel infection mechanism.

The paper shows that MxB's N-terminal domain binds to herpesvirus capsids and is packaged into virions, restricting HSV-1 and PrV infections.

The paper integrates structural and proteomic methods to characterize the protein organization of a giant virus, identifying sub-compartments and membrane protein topologies.

The paper develops a mass spectrometry method to quantify inositol pyrophosphate-protein interactions on a proteome-wide scale, identifying new pyrophosphorylated proteins and highlighting Mg2+ effects on binding.

The paper identifies mTOR's role in regulating developmental gene promoters in pluripotent mouse embryonic stem cells through interaction with PRC1 and RNA Polymerase II pausing.

This study shows MTMR14 is recruited to damaged lysosomes to remodel lipids, reduce mTORC1 signaling, and support proteostasis during lysosomal injury.

The paper identifies a direct interaction between postsynaptic proteins and ribosomes, revealing a localized synaptic translatome that preferentially translates PSD-related mRNAs.

This study shows that increased somatodendritic Tau interacts with nuclear envelope proteins, causing nuclear damage and altering gene expression related to AD and tauopathies.

The paper uses integrative structural biology to model the human Mic60-Mic19 subcomplex and validate its role as a diffusion barrier in mitochondrial crista junctions.

This study shows that Bruchpilot remodeling couples synaptic structure to Kv1/Shaker channel control of excitability, restoring function in sleepless mutants.

This paper structurally characterizes CCDC127 as a mitochondrial intermembrane space protein involved in the mitoskeleton framework.

This study identifies PLPPR3 condensates as actin sinks that locally boost monomer concentrations, promoting actin polymerization and filopodia formation in neurons.

This study identifies oligophosphorylation as a new phosphorylation mode, showing NME1 can catalyze its own oligophosphorylation using ATP, with implications for phosphoregulation.

This study shows that autophagy in Drosophila mushroom bodies regulates synaptic architecture and sleep resilience by modulating presynaptic active zone proteins and triggering a systemic proteostatic stress response.

The paper audits error control in twelve crosslinking mass spectrometry pipelines using ground-truth designs, finding some pipelines exceed false discovery rates by up to 29-fold.

The paper uses integrative molecular dynamics simulations to analyze cross-linking mass spectrometry data and reveal mitochondrial protein distributions, including the localization of FAM162A and TMEM126A in cristae.

This study uses cryo-electron tomography to describe the molecular architecture and structural heterogeneity of individual synaptic vesicles in mouse brains and cultured neurons.

The paper develops recombinant protein standards and a search engine for XL-MS, showing Scout outperforms existing tools in speed, sensitivity, and false-discovery rate control.

This study identifies mitochondrial adaptations in naked mole-rats that confer hypoxia resistance, linking social behavior to molecular evolution.

This paper introduces CLASP, a cross-link assisted spatial proteomics method, to map sub-organelle proteomes and membrane protein topology in human mitochondria.

This study identifies Blobby as a novel regulator of presynaptic active zone nanopatterning and shows its role in maintaining Ca2+ channel packing density and synaptic function.

This study compares four lysis buffers for proteomics, finding SDS most reliable and advocating a dual-sample approach to maximize proteome coverage.

This study identifies an antagonistic interaction between Spinophilin and Syd-1 that regulates presynaptic long-term plasticity and olfactory memory in Drosophila.

The paper introduces real-time library search (RTLS) to improve cross-link identification in XL-MS by leveraging intensity ratios of diagnostic peaks, enhancing identification depth in complex samples.