The B. subtilis replicative polymerases bind the sliding clamp with different strengths to tune replication processivity and fidelity
Luke G. O’Neal, Madeline N. Drucker, Ngoc Khanh Lai, Ashley F. Clemente, Alyssa P. Campbell, Lindsey E. Way, Sinwoo Hong, Emily E. Holmes, Sarah J. Rancic, Nicholas Sawyer, Xindan Wang, Elizabeth S. Thrall
This study investigates how the sliding clamp interacts with two replicative polymerases in B. subtilis, showing that PolC-DnaN binding is essential for replication while DnaE-DnaN interaction is dispensable but affects mutagenesis.
bioRxiv · Mar 10, 2025 · PreprintBiochemistry