Direct readout of neural stem cell transgenesis with an integration-coupled gene expression switch
Takuma Kumamoto, Franck Maurinot, Raphaëlle Barry‐Martinet, Célia Vaslin, Sandrine Vandormael‐Pournin, Mickaël Le, Marion Lerat, Dragoş Niculescu, Michel Cohen‐Tannoudji, Alexandra Rebsam, Karine Loulier, Stéphane Nedelec, Samuel Tozer, Jean Livet
This paper introduces a genetic switch called iOn that enables the identification of genomic integration events by triggering gene expression upon insertion, improving transgenesis in stem cells and model organisms.
Neuron · Jun 18, 2020Peer-reviewedDevelopmental Biology