A portable orthogonal replication system enables continuous gene evolution near the biological speed limit
Rongzhen Tian, Fabian B. H. Rehm, Matthew Kenneth, Kiarash Jamali, Petar S. Zhotev, Kim C. Liu, Jason W. Chin
This paper introduces an improved orthogonal replication system in E. coli that enables rapid gene evolution with high mutation rates and demonstrates its application in evolving ethanol assimilation pathways.
bioRxiv · Feb 24, 2026 · PreprintSynthetic Biology