The E. coli Cas1/2 endonuclease complex reduces CRISPR/Cascade guide array stability
Zhixia Ye, Eirik A. Moreb, Shuai Li, Juliana Lebeau, Romel Menacho‐Melgar, Matthew Munson, Michael Lynch
The paper shows that the Cas1/2 endonuclease complex in E. coli causes instability in CRISPR/Cascade guide arrays, and controlled expression of the Cascade operon improves stability.
ACS Synthetic Biology · Dec 17, 2020Peer-reviewedSynthetic Biology