Zhixia Ye

The Development of 2-stage Microfermentation Protocols for High Throughput Cell Factory Evaluations

The paper develops 2-stage microfermentation protocols for high-throughput evaluation of engineered E. coli strains for producing various products.

Two-stage Dynamic Deregulation of Metabolism Improves Process Robustness & Scalability in Engineered E. coli.

This paper demonstrates that dynamic deregulation of central metabolism in E. coli improves process robustness and scalability for industrial chemical production.

"Multi-Agent" Screening Improves the Efficiency of Directed Enzyme Evolution

This paper introduces a multi-agent directed evolution approach that identifies hidden mutations by using multiple substrates, improving enzyme engineering efficiency.

The E. coli Cas1/2 endonuclease complex reduces CRISPR/Cascade guide array stability

The paper shows that the Cas1/2 endonuclease complex in E. coli causes instability in CRISPR/Cascade guide arrays, and controlled expression of the Cascade operon improves stability.

Improved, scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion.

This paper describes an improved method for recombinant protein expression in E. coli using phosphate depletion to trigger autoinduction, achieving high protein titers across various scales.

Robustness testing and scalability of phosphate regulated promoters useful for two-stage autoinduction in E. coli

The paper tests the robustness of PhoB-regulated promoters in E. coli across different media and identifies promoters with consistent expression for two-stage autoinduction.