Romel Menacho‐Melgar

This paper describes a systems engineering approach to improve the scalable and robust production of nanobodies in E. coli through dynamic control of reduction potential and two-stage purification.

The paper develops 2-stage microfermentation protocols for high-throughput evaluation of engineered E. coli strains for producing various products.

This study reviews 168 publications on protein precipitation and liquid-liquid extraction, finding these methods are not always cheaper than chromatography and require optimization for cost-effectiveness.

This paper describes an improved bioprocess for producing Q-Griffithsin, a clinical-stage antiviral, using integrated autolysis, DNA hydrolysis, and precipitation to achieve high purity and yield without conventional chromatography.

This paper describes a method for rapidly producing and purifying Taq polymerase using engineered E. coli strains with autoinducible expression and simplified purification steps.

The paper shows that the Cas1/2 endonuclease complex in E. coli causes instability in CRISPR/Cascade guide arrays, and controlled expression of the Cascade operon improves stability.

This paper describes a method to improve protein expression and purification in E. coli by inducing autolysis and DNA/RNA hydrolysis via phage lysozyme and endonuclease, achieving >90% protein release.

This paper describes an improved method for recombinant protein expression in E. coli using phosphate depletion to trigger autoinduction, achieving high protein titers across various scales.

The paper tests the robustness of PhoB-regulated promoters in E. coli across different media and identifies promoters with consistent expression for two-stage autoinduction.