Eirik A. Moreb

The Development of 2-stage Microfermentation Protocols for High Throughput Cell Factory Evaluations

The paper develops 2-stage microfermentation protocols for high-throughput evaluation of engineered E. coli strains for producing various products.

A meta-analysis of gRNA library screens enables an improved understanding of the impact of gRNA folding and structural stability on CRISPR-Cas9 activity.

The paper analyzes CRISPR-Cas9 datasets to identify two inhibitory secondary structures in gRNAs and provides free energy cutoffs to guide future gRNA design.

Two-stage Dynamic Deregulation of Metabolism Improves Process Robustness & Scalability in Engineered E. coli.

This paper demonstrates that dynamic deregulation of central metabolism in E. coli improves process robustness and scalability for industrial chemical production.

An Analysis of gRNA Sequence Dependent Cleavage Highlights the Importance of Genomic Context on CRISPR-Cas Activity

The paper examines how genomic context affects gRNA activity across species and highlights limitations of current gRNA prediction algorithms.

The E. coli Cas1/2 endonuclease complex reduces CRISPR/Cascade guide array stability

The paper shows that the Cas1/2 endonuclease complex in E. coli causes instability in CRISPR/Cascade guide arrays, and controlled expression of the Cascade operon improves stability.

Improved, two-stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease

This paper describes a method to improve protein expression and purification in E. coli by inducing autolysis and DNA/RNA hydrolysis via phage lysozyme and endonuclease, achieving >90% protein release.

Improved, scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion.

This paper describes an improved method for recombinant protein expression in E. coli using phosphate depletion to trigger autoinduction, achieving high protein titers across various scales.

Robustness testing and scalability of phosphate regulated promoters useful for two-stage autoinduction in E. coli

The paper tests the robustness of PhoB-regulated promoters in E. coli across different media and identifies promoters with consistent expression for two-stage autoinduction.