Genome editing of phylogenetically distinct bacteria using portable retron-mediated recombineering
Alejandro González-Delgado, Laura Bonillo-Lopez, Milo S. Johnson, Nastassia Knödlseder, Ching‐Chung Ko, Yassir Lekbach, Jee‐Hwan Oh, Hemaa Selvakumar, Michael C. Wold, Zihan Yu, Virginia Aragón, Jeffrey A. Gralnick, Marc Güell, Graham F. Hatfull, Benjamin K. Keitz, , Seth L. Shipman
This study demonstrates that retron-mediated recombineering can be applied across multiple bacterial species, achieving high editing efficiencies in several phyla.
bioRxiv · Jun 16, 2025 · PreprintBioengineering