Author

Alejandro González-Delgado

Gladstone InstitutesORCID 0000-0002-8357-3204Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:bacteria (other)· 2 Approach:experimental assay· 2 Research Focus:benchmarking· 1 Research Focus:cell type annotation· 1 Assay:DNA methylation· 1 Organism:E. coli· 1 Research Focus:gene regulatory network inference· 1 Research Focus:genome assembly· 1

Frequent Co-Authors

Seth L. ShipmaniD· 2 shared Ana Dávila-Hidalgo· 1 shared Arturo CarabiasiD· 1 shared Benjamin K. KeitziD· 1 shared Britt Louise KoskellaiD· 1 shared Ching‐Chung KoiD· 1 shared Darshini Poola· 1 shared Dennis J. ZhangiD· 1 shared

Activity by Year

2025-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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2 shared papersMicrobiology

Seth L. ShipmanORCID

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2 papers

Recent·Most cited

Reverse transcribed ssDNA derepresses translation of a retron antiviral protein

Karen Huilin Zhang, Matías Rojas-Montero, Darshini Poola, Josepha Magdalene Klas, Arturo Carabias, Dennis J. Zhang, Mario Rodríguez Mestre, Ruiliang Zhao, Ana Dávila-Hidalgo, Guillermo Montoya, Rafael Pinilla‐Redondo, Alejandro González-Delgado, Seth L. Shipman

The paper describes a non-canonical mechanism in Type VI retrons where reverse transcribed ssDNA derepresses translation of an antiviral toxin, providing phage defense without detectable msDNA.

bioRxiv · Oct 22, 2025 · PreprintMicrobiology

Genome editing of phylogenetically distinct bacteria using portable retron-mediated recombineering

Alejandro González-Delgado, Laura Bonillo-Lopez, Milo S. Johnson, Nastassia Knödlseder, Ching‐Chung Ko, Yassir Lekbach, Jee‐Hwan Oh, Hemaa Selvakumar, Michael C. Wold, Zihan Yu, Virginia Aragón, Jeffrey A. Gralnick, Marc Güell, Graham F. Hatfull, Benjamin K. Keitz, , Seth L. Shipman

This study demonstrates that retron-mediated recombineering can be applied across multiple bacterial species, achieving high editing efficiencies in several phyla.

bioRxiv · Jun 16, 2025 · PreprintBioengineering