DNA-guided CRISPR/Cas12 for RNA targeting
Carlos Orosco, Santosh R. Rananaware, Boyu Huang, Michael P. Hanna, M. Reza Ahmadimashhadi, Jordan G. Lewis, Michael P. Baugh, August P. Bodin, Sarah J. Flannery, Ian H. Lange, Zoe R. Fang, Vedant N. Karalkar, Katelyn S. Meister, Piyush Jain
This study introduces {Psi}DNA, a DNA-based guide for Cas12 enzymes that enables specific and efficient RNA targeting, achieving 100% accuracy in detecting HCV RNA.
medRxiv · Nov 21, 2024 · PreprintGenetic & Genomic Medicine