Biotin-operated ligand trapping enables dynamic spatial redistribution of protein and organelle in living cells
Wyatt C. Beyers, Sarah E. Plutkis, Kathryn R Rutcher, Julianna L. Sun, MaryEllen R. Haas, Mollie S. Hutton, Hark Kyun Kim, Alejandro Chavez, Shondra M. Pruett‐Miller, Jason Vevea, Luke D. Lavis, Chi‐Lun Chang
This paper introduces BOLT, a tool for inducible trapping, controlled release, and visualization of proteins in living cells, demonstrating its use in tracking protein and organelle dynamics.
bioRxiv · Oct 6, 2026 · PreprintSynthetic Biology