Alejandro Chavez

This paper introduces BOLT, a tool for inducible trapping, controlled release, and visualization of proteins in living cells, demonstrating its use in tracking protein and organelle dynamics.

This paper introduces a reconfigurable microscopy module that combines HIST and line-scanning confocal techniques for versatile fluorescence imaging.

This study identifies eIF4G2 as a translational checkpoint that restricts pancreatic cancer progression by maintaining epithelial identity and limiting metastasis.

This study identifies genetic regulators of microglial inflammation in Alzheimer's disease using CRISPRi screens and scRNA-seq, revealing key genes that modulate inflammatory states and disease-relevant clusters.

This paper develops a CRISPR-guided transposon system for DSB-free DNA integration in human cells, improving efficiency and reducing off-target effects.

The paper develops a multiplex reverse genetics platform to identify genetic modifiers of protein misfolding in neurodegenerative diseases and identifies DNAJB6 as a modifier of toxicity and solubility of ALS/FTD-linked RNA-binding proteins.

The paper uses CRISPRi and single-cell RNA-seq to reverse engineer gene regulatory networks in colorectal cancer cells, revealing autoregulatory and cooperative mechanisms.

ampliCan provides precise quantification and visualization of genome editing events, outperforming existing tools in benchmark tests.

This paper introduces daisy-chain gene drives that can alter local populations without global spread, enabling ethical use and community control.

This paper shows that RNA polymerase can dislodge Cas9 from DNA breaks, improving genome editing and bacterial immunity by increasing mutagenesis and phage resistance.

The paper designs a compact CRISPR-Cas9 activator suitable for AAV-mediated delivery and validates its transcriptional activation efficacy in multiple cell lines.

This paper introduces a method to tune guide RNA for precise Cas9 targeting, preventing mutations in engineered organisms and demonstrating scalability and robustness across Cas9 variants and environments.

The paper introduces a Cas9-based method to create and functionally profile eukaryotic DNA variant libraries, enabling efficient analysis of genetic alterations.

This study demonstrates RNA-guided gene drives in yeast can efficiently and reversibly bias inheritance when mated to wild-type organisms.

This study develops a highly efficient Cas9-based transcriptional activator, VPR-Cas9, capable of activating multiple genes and promoting neuronal differentiation from iPSCs.