Choice of lipid supplementation for in vitro erythroid cell culture impacts reticulocyte yield and characteristics
C. M. Freire, N. R. King, Monika Dzieciątkowska, Daniel Stephenson, Johannes G. G. Dobbe, Geert J. Streekstra, A. D’Alessandro, Timothy J. Satchwell, Ashley Mark Toye
This study compares lipid sources in erythroid cultures, finding that detergent-treated plasma leads to cholesterol-deficient reticulocytes with impaired function, while AB-serum supports stable reticulocyte generation.
Scientific Reports · Jan 29, 2026Peer-reviewedCell Biology