Real-time affinity measurements of proteins synthesized in cell-free lysate using fluorescence correlation spectroscopy
Chao Liu, Steven A. Hoang-Phou, Congwang Ye, Emma J. Laurence, Matthew J. Laurence, Erika J. Fong, Nikki M. Hammond, B. Dillon Vannest, Nicholas N. Watkins, Ted A. Laurence, Matthew A. Coleman
This paper develops a method to measure protein-protein interactions in real-time using cell-free synthesis and fluorescence correlation spectroscopy, achieving high sensitivity and throughput.
Analytical Chemistry · Apr 30, 2025Peer-reviewedBiophysics