Noémi Becza

Assessing the affinity spectrum of an antigen-specific memory B cell repertoire by inverted ImmunoSpot

This paper introduces a high-throughput method to assess the affinity distribution of antigen-specific B cells using an adapted ImmunoSpot assay, demonstrating its application in detecting affinity maturation after vaccination.

The magnitude of the secondary B cell response is primarily defined by antibody feedback inhibition rather than the number of memory B cells present

The study shows that the secondary B cell response magnitude is driven by antibody feedback inhibition, not the number of memory B cells before vaccination.