NMR measurements of transient low-populated tautomeric and anionic Watson-Crick-like G·T/U in RNA:DNA hybrids: Implications for the fidelity of transcription and CRISPR/Cas9 gene editing
O. Szekely, A. K. Rangadurai, S. Gu, A. Manghrani, S. Guseva, H. Al-Hashimi
This study uses NMR to show that G*T/U mismatches in RNA:DNA hybrids can form Watson-Crick-like conformations, potentially contributing to transcription errors and CRISPR/Cas9 off-target effects.
bioRxiv · Aug 24, 2023Biophysics