P. Poole

Pisum sativum applies sanctions to less effective strains at the nodule level, but not within mixed nodules

This study shows that pea plants sanction entire nodules based on nitrogen fixation rates, not individual bacteria, preventing ineffective strains from evading sanctions by piggybacking.

Barley cultivars shape the abundance, phenotype, genotype and gene expression of their associated microbiota by differential root exudate secretion

Factors governing attachment of Rhizobium leguminosarum to legume roots

This study identifies 115 proteins required for Rhizobium leguminosarum attachment to pea roots under varying pH conditions, revealing complex mechanisms and no single essential protein for alkaline attachment.

Control of N2 fixation and NH3 excretion in Azorhizobium caulinodans ORS571

This paper engineers Azorhizobium caulinodans to control N2 fixation and NH3 excretion using rhizopine-inducible systems to target nitrogen delivery to transgenic crops.

A simple assay for quantification of plant-associative bacterial nitrogen fixation

This paper introduces a modified in planta acetylene reduction assay to accurately quantify plant-associative bacterial nitrogen fixation in barley roots.

Lifestyle adaptations of Rhizobium from rhizosphere to symbiosis

This study identifies genes critical for different stages of Rhizobium-legume symbiosis using INSeq, showing 603 genes are required for nodulation and N2 fixation, with 146 shared across stages.

A Bacterial Expression Vector Archive (BEVA) forflexible modular assembly of Golden Gate-compatible vectors

The paper introduces a modular bacterial vector assembly system using Esp3I for Golden Gate cloning, demonstrating three example vectors for gene expression in Rhizobium leguminosarum and environmental samples.