Structure of Geobacillus stearothermophilus Cas9: insights into the catalytic process and thermostability of CRISPR-Cas9
Panpan Shen, Lilan Zhang, Beibei Liu, Xian Li, Caihong Wang, Jian Min, Jian‐Wen Huang, Chun‐Chi Chen, Rey‐Ting Guo
This study reports the cryo-EM structures of Geobacillus stearothermophilus Cas9 complexed with sgRNA and target DNA, revealing the catalytic mechanism and thermal stability features.
ACS Catalysis · Aug 20, 2024Peer-reviewedMolecular Biology