The paper structures and engineers deep-sea halophilic PET hydrolases to improve their salt tolerance and catalytic efficiency for industrial applications.

中国科学通报：英文版 · Sep 1, 2026Peer-reviewedMolecular Biology

Enabling regiospecific di-halogenation in one-pot reactions using an engineered single-component flavin-dependent tryptophan halogenase

Hao Li, Jian‐Wen Huang, Si Dai, Deyi Feng, J. Liu, Nan Zhang, Yaojie Guo, Chun‐Chi Chen, Rey‐Ting Guo

The paper engineers a single-component flavin-dependent tryptophan halogenase to enable regiospecific mono- and di-halogenation of tryptophan in one-pot reactions.

ACS Catalysis · Dec 5, 2025Peer-reviewedMolecular Biology

Full-length structure of CPR-containing self-sufficient cytochrome P450

Zhenzhen Xie, Ziwei Liu, Siyu Li, Kangwei Xu, Jian‐Wen Huang, Jian Min, Q. T. Li, Jingxue Zhai, Te Wang, Yutong Wang, Lu Yang, Junjie Duan, Junjie Chen, Ruibo Wu, Chun-Chi Chen, Rey‐Ting Guo

The paper reports the structure of SoP450, a self-sufficient cytochrome P450, revealing its dimeric form and electron transfer mechanism.

bioRxiv · Nov 29, 2025 · PreprintSynthetic Biology

Structure of Geobacillus stearothermophilus Cas9: insights into the catalytic process and thermostability of CRISPR-Cas9

Panpan Shen, Lilan Zhang, Beibei Liu, Xian Li, Caihong Wang, Jian Min, Jian‐Wen Huang, Chun‐Chi Chen, Rey‐Ting Guo

This study reports the cryo-EM structures of Geobacillus stearothermophilus Cas9 complexed with sgRNA and target DNA, revealing the catalytic mechanism and thermal stability features.

ACS Catalysis · Aug 20, 2024Peer-reviewedMolecular Biology