Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants
Ramona Grützner, Patrick Martin, Claudia Horn, Samuel Breselge, Erin J. Cram, Carolyn W. T. Lee‐Parsons, Johannes Stuttmann, Sylvestre Marillonnet
This study shows that adding 13 introns to the Cas9 gene significantly improves gene knockout efficiency in plants.
bioRxiv · Apr 3, 2020 · PreprintMolecular Biology