Author

Ramona Grützner

Leibniz Institute of Plant Biochemistry

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Molecular Biology· 4

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Organism:plant (other)· 3 Assay:CRISPR screen· 2 Research Focus:perturbation response prediction· 2 Organism:Arabidopsis· 1 Research Focus:gene regulatory network inference· 1 Research Focus:protein design· 1 Assay:protein sequence· 1

Frequent Co-Authors

Sylvestre MarillonnetiD· 4 shared Alain F. TissieriD· 2 shared Anja Prange· 2 shared Claudia HorniD· 2 shared Thomas Iwen· 2 shared Tom SchreiberiD· 2 shared Alex OlengoiD· 1 shared Ann NantezaiD· 1 shared

Activity by Year

2020-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 23 mutual co-author links

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4 shared papersMolecular Biology

Sylvestre MarillonnetORCID

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4 papers

Recent·Most cited

Efficient scar-free knock-ins of several kilobases by engineered CRISPR/Cas endonucleases

Tom Schreiber, Anja Prange, Petra Schäfer, Thomas Iwen, Ramona Grützner, Sylvestre Marillonnet, Alain F. Tissier

The paper demonstrates that fusing CRISPR endonucleases to specific 5'-exonucleases significantly increases scar-free multikilobase knock-in frequencies in plants.

bioRxiv · Nov 24, 2023 · PreprintMolecular Biology

Molecular cloning and recombinant protein expression of 9-Lipoxygenase gene from Solanum tuberosum in Pichia pastoris yeast cells

Alex Olengo, Ann Nanteza, Elvio Henrique Benatto Perino, Leif‐Alexander Garbe, Sylvestre Marillonnet, Ramona Grützner, Margaret Saimo-Kahwa, Fabien Schultz, Matthias Koch

This study clones and expresses a 9-lipoxygenase gene from Solanum tuberosum in Pichia pastoris to produce recombinant protein for biodegradable fungicide development.

bioRxiv · May 18, 2022 · PreprintMolecular Biology

DNA double strand breaks lead to de novo transcription and translation of damage-induced long RNAs in planta

Tom Schreiber, Sunita Tripathee, Thomas Iwen, Anja Prange, Khabat Vahabi, Ramona Grützner, Claudia Horn, Sylvestre Marillonnet, Alain F. Tissier

This study shows that DNA double strand breaks (DSBs) in plants induce the transcription and translation of damage-induced long RNAs (dilRNAs) into proteins.

bioRxiv · May 11, 2022 · PreprintMolecular Biology

Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants

Ramona Grützner, Patrick Martin, Claudia Horn, Samuel Breselge, Erin J. Cram, Carolyn W. T. Lee‐Parsons, Johannes Stuttmann, Sylvestre Marillonnet

This study shows that adding 13 introns to the Cas9 gene significantly improves gene knockout efficiency in plants.

bioRxiv · Apr 3, 2020 · PreprintMolecular Biology