Rational engineering of allosteric protein switches by in silico prediction of domain insertion sites
Benedict Wolf, Pegi Shehu, Luca Brenker, Adrian Bachmann, Ann-Sophie Helene Kroell, Nicholas T. Southern, Stefan Holderbach, Joshua Eigenmann, Sabine Aschenbrenner, Jan Mathony, Dominik Niopek
This paper introduces ProDomino, a machine learning tool for predicting domain insertion sites in proteins, validated experimentally in E. coli and human cells to create opto- and chemogenetic switches.
bioRxiv · Dec 4, 2024 · PreprintSynthetic Biology