Benedict Wolf

This paper develops a phage-assisted evolution platform to engineer allosteric protein switches, achieving near-binary optogenetic switches with high dynamic range.

This paper engineers thermo-responsive allosteric proteins using temperature-sensitive domains, enabling precise control of protein activity in both bacterial and mammalian systems.

This paper introduces ProDomino, a machine learning tool for predicting domain insertion sites in proteins, validated experimentally in E. coli and human cells to create opto- and chemogenetic switches.

This paper develops a versatile platform for optogenetic and chemogenetic control of CRISPR-Cas9 and Cas12 across multiple orthologs using engineered anti-CRISPR proteins.

This paper inverts the AlphaFold2 network to perform de novo protein design, showing that while the method generates viable sequences with correct folds, it requires post-design optimization to achieve optimal surface properties.