Sabine Aschenbrenner

De novo design of light-controlled conformational protein switches

This paper computationally designs light-responsive proteins that undergo conformational changes upon light activation, enabling precise control of biological functions.

Modular Engineering of Thermo-Responsive Allosteric Proteins

This paper engineers thermo-responsive allosteric proteins using temperature-sensitive domains, enabling precise control of protein activity in both bacterial and mammalian systems.

Rational engineering of allosteric protein switches by in silico prediction of domain insertion sites

This paper introduces ProDomino, a machine learning tool for predicting domain insertion sites in proteins, validated experimentally in E. coli and human cells to create opto- and chemogenetic switches.

A modular toolbox for the optogenetic deactivation of transcription

The paper introduces LOOMINA, an optogenetic system that deactivates transcriptional activation in response to blue light, offering high adaptability and compatibility with various effector proteins.

A Versatile Anti-CRISPR Platform for Opto- and Chemogenetic Control of CRISPR-Cas9 and Cas12 across a Wide Range of Orthologs

This paper develops a versatile platform for optogenetic and chemogenetic control of CRISPR-Cas9 and Cas12 across multiple orthologs using engineered anti-CRISPR proteins.

A deep mutational scanning platform to characterize the fitness landscape of anti-CRISPR proteins

The paper develops a deep mutational scanning pipeline for anti-CRISPR proteins in E. coli, revealing their mutational tolerance and redundancy, and validates findings in mammalian cells.

Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein

This paper introduces an optogenetic tool to control NmeCas9 activity using a light-switchable anti-CRISPR protein, enabling precise genome editing in mammalian cells.

Computational design of anti-CRISPR proteins with improved inhibition potency and expanded specificity

The paper designs improved anti-CRISPR proteins with enhanced inhibition potency and expanded specificity through structure-based engineering.

Cell-specific CRISPR/Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins

This paper develops a cell-specific CRISPR/Cas9 activation system using miRNA-regulated anti-CRISPR proteins, demonstrating targeted gene activation in liver and muscle cells.