Nicholas T. Southern

The paper inserts bulky domains into a CRISPR adenine base editor to narrow its editing window and reduce off-target effects.

This paper develops a phage-assisted evolution platform to engineer allosteric protein switches, achieving near-binary optogenetic switches with high dynamic range.

This paper engineers thermo-responsive allosteric proteins using temperature-sensitive domains, enabling precise control of protein activity in both bacterial and mammalian systems.

This paper introduces ProDomino, a machine learning tool for predicting domain insertion sites in proteins, validated experimentally in E. coli and human cells to create opto- and chemogenetic switches.

The paper introduces LOOMINA, an optogenetic system that deactivates transcriptional activation in response to blue light, offering high adaptability and compatibility with various effector proteins.

This paper improves CRISPR/dCas9 interference for neuronal gene suppression by using a dual lentivirus system and dCas9-KRAB-MeCP2 construct, showing superior repression of the Bdnf gene in rat neurons.