Ann-Sophie Helene Kroell

De novo design of light-controlled conformational protein switches

This paper computationally designs light-responsive proteins that undergo conformational changes upon light activation, enabling precise control of biological functions.

Phage-Assisted Evolution of Allosteric Protein Switches

This paper develops a phage-assisted evolution platform to engineer allosteric protein switches, achieving near-binary optogenetic switches with high dynamic range.

Modular Engineering of Thermo-Responsive Allosteric Proteins

This paper engineers thermo-responsive allosteric proteins using temperature-sensitive domains, enabling precise control of protein activity in both bacterial and mammalian systems.

Rational engineering of allosteric protein switches by in silico prediction of domain insertion sites

This paper introduces ProDomino, a machine learning tool for predicting domain insertion sites in proteins, validated experimentally in E. coli and human cells to create opto- and chemogenetic switches.