Accuracy, Reproducibility And Bias Of Next Generation Sequencing For Quantitative Small RNA Profiling: A Multiple Protocol Study Across Multiple Laboratories
MD Giraldez, Ryan M. Spengler, Alton Etheridge, PM Godoy, Andrea J. Barczak, Srimeenakshi Srinivasan, PL De Hoff, Kahraman Tanrıverdi, Amanda Courtright, Shu Lu, Joseph A. Khoory, R. Rubio, David H. Baxter, Tom A. P. Driedonks, HPJ Buermans, , Muneesh Tewari
This study evaluates the accuracy, reproducibility, and bias of small RNA-seq across five protocols and nine labs, finding protocol-specific bias that can be mitigated with adapters and computational corrections.
bioRxiv · May 17, 2017 · PreprintGenomics