Esther N. M. Nolte‐‘t Hoen

Development of a live-cell imaging assay to elucidate spatiotemporal dynamics of extracellular vesicle fusion with target cells

The paper develops a live-cell imaging assay to visualize and quantify extracellular vesicle fusion with target cells, enabling study of spatiotemporal dynamics and molecular regulators of EV fusion.

EVtrace: tracing extracellular vesicles-associated proteins in recipient cells using stable isotope labeling

EVtrace uses SILAC to trace EV-associated proteins in recipient cells, showing non-stochastic targeting of EV subpopulations to specific cell types.

Sphingomyelins in mosquito saliva modify the host lipidome to enhance transmission of flaviviruses by promoting viral protein levels

This study identifies sphingomyelins in mosquito salivary extracellular vesicles that enhance flavivirus transmission by altering the host lipidome and increasing viral protein levels.

Accuracy, Reproducibility And Bias Of Next Generation Sequencing For Quantitative Small RNA Profiling: A Multiple Protocol Study Across Multiple Laboratories

This study evaluates the accuracy, reproducibility, and bias of small RNA-seq across five protocols and nine labs, finding protocol-specific bias that can be mitigated with adapters and computational corrections.