Differences in the chitinolytic activity of mammalian chitinases on soluble and crystalline substrates
B. A. Barad, L. Liu, R. E. Diaz, R. Basilio, S. J. Van Dyken, R. M. Locksley, J. S. Fraser
This study identifies mutations that enhance chitinase activity on oligomeric substrates but not crystalline chitin, highlighting the importance of using physiologically relevant substrates in enzyme engineering.
bioRxiv · Oct 17, 2019 · PreprintBiochemistry