Identifying phenotype-genotype-function coupling in 3D organoid imaging using Shape, Appearance and Motion Phenotype Observation Tool (SPOT)
Felix Zhou, Brittany‐Amber Jacobs, Adam Norton-Steele, Xiaoyue Han, Linna Zhou, Thomas M. Carroll, Carlos Ruiz Puig, Joseph Chadwick, Xiao Qin, Richard Lisle, Lewis Marsh, Helen Mary Byrne, Heather Harrington, Xin Lü
The paper develops SPOT, a tool for analyzing 3D morpho-dynamics from 2D live-cell imaging, and applies it to organoids to identify phenotype-genotype-function relationships.
Nature Communications · Aug 17, 2026Peer-reviewedCancer Biology