Reading frame restoration at the EYS locus, and allele-specific chromosome removal after Cas9 cleavage in human embryos
Michael V. Zuccaro, Jia L. Xu, Carl A. Mitchell, Diego Marín, Raymond Zimmerman, Bhavini Rana, Everett Weinstein, Rebeca T. King, Katherine L. Palmerola, Morgan Elizabeth Smith, Stephen H. Tsang, Robin Goland, Maria Jasin, Rogerio A. Løbo, Nathan R. Treff, Dieter M. Egli
The paper evaluates Cas9-induced repair outcomes at the EYS locus in human embryos, showing microhomology-mediated end joining restores reading frames without mosaicism, while unrepaired breaks lead to chromosome loss.
Cell · Oct 29, 2020Peer-reviewedGenetics