Jia L. Xu

This study shows that DNA double-strand breaks in human embryos lead to chromosome loss through sister chromatid tethering and centromere-mediated separation.

This study shows that base editing in human embryos produces efficient on-target changes without chromosomal abnormalities or large deletions, unlike CRISPR/Cas9-induced DSBs.

This study shows that CRISPR-Cas9 editing in human embryos is limited by slow DSB repair kinetics, leading to mosaicism and chromosomal abnormalities.

The paper evaluates Cas9-induced repair outcomes at the EYS locus in human embryos, showing microhomology-mediated end joining restores reading frames without mosaicism, while unrepaired breaks lead to chromosome loss.