Michael V. Zuccaro

This study shows that a low nucleus-to-cytoplasmic ratio in haploid embryonic cells leads to cytokinesis failure, genome instability, and reduced development.

The paper shows that DNA replication patterns in early mammalian embryos are established early, with late replicating regions near the nuclear lamina showing fragility and predisposing long genes to genetic changes.

The paper profiles DNA replication timing in haploid and diploid human ESCs, identifying delayed replication in autosomal regions and linking it to X chromosome dosage and polyploid cell underreplication.

The paper evaluates Cas9-induced repair outcomes at the EYS locus in human embryos, showing microhomology-mediated end joining restores reading frames without mosaicism, while unrepaired breaks lead to chromosome loss.