Ryan Marshall

In vitro implementation of robust gene regulation in a synthetic biomolecular integral controller

The paper designs and validates an in vitro synthetic gene circuit that uses integral feedback to maintain gene expression at a target level despite disturbances.

Distinct timescales of RNA regulators enable the construction of a genetic pulse generator

The paper characterizes two RNA regulators with distinct timescales and demonstrates their use in creating a genetic pulse generator through computational modeling and experimental validation.

Rapid and scalable characterization of CRISPR technologies using an E. coli cell-free transcription-translation system

This paper introduces an E. coli cell-free system to rapidly and scalably characterize CRISPR technologies, including DNA cleavage, gene repression, and anti-CRISPR activity.

Tuning of recombinant protein expression in Escherichia coli by manipulating transcription, translation initiation rates and incorporation of non-canonical amino acids

This study investigates how altering transcription and translation rates in E. coli affects protein expression, revealing that increased initiation rates can reduce expression and that codon bias helps regulate synthesis.