Efficient gene disruption with CRISPR-Cas3 in human T cells
Tomoaki Fujii, Yukimi Sakoda, Kazuto Yoshimi, Kohei Takeshita, Shôji Watanabe, Ryuya Iida, Tsubasa Obo, Kazumasa Yokoyama, Koji Tamada, Tomoji Mashimo
This study demonstrates that CRISPR-Cas3 can efficiently and safely disrupt genes in human T cells, reducing off-target effects and alloreactivity while maintaining cytotoxicity.
NAR Cancer · Mar 26, 2026Peer-reviewedCancer Biology