Kohei Takeshita

This paper develops a CRISPR-Cas3-based diagnostic platform for emerging RNA viruses, enabling rapid assay design and deployment with high sensitivity and portability.

This study demonstrates that CRISPR-Cas3 can efficiently and safely disrupt genes in human T cells, reducing off-target effects and alloreactivity while maintaining cytotoxicity.

This study determines the crystal structure of BCG3185c and identifies a large pocket near its catalytic center that may bind agelasine D, suggesting its role in inhibiting Mycobacterium tuberculosis growth.

This study elucidates the mechanism of CRISPR-Cas3-mediated DNA degradation in E. coli, showing how EcoCas3 cleaves ssDNA and degrades dsDNA through specific and non-specific interactions with EcoCascade.