A New Platform for Label-Free, Proximal Cellular Pharmacodynamic Assays: Identification of Glutaminase Inhibitors Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry
F. Pu, A. J. Radosevich, B. G. Bruckner, D. A. Fontaine, S. C. Panchal, J. D. Williams, S. M. Gopalakrishnan, N. L. Elsen
This paper introduces a high-throughput label-free cellular pharmacodynamic assay using IR-MALDESI to identify glutaminase inhibitors and confirm hits with mass spectrometry-based assays.
bioRxiv · Jan 30, 2023Biochemistry