Efficient protein tagging and cis-regulatory element engineering via precise and directional oligonucleotide-based targeted insertion in plants
J. Kumar, S. N. Char, T. Weiss, H. Liu, B. Liu, B. Yang, F. Zhang
This study improves targeted insertion in plants using chemically protected dsODNs with 5' 1-nt overhangs, achieving directional and precise insertions for gene tagging and cis-regulatory engineering in Setaria viridis and rice.
bioRxiv · Apr 20, 2023 · PreprintPlant Biology