W. B. Frommer

Loss-of-function mutation in the polyamine transporter gene OsLAT5 as a selectable marker for genome editing

The paper demonstrates that loss-of-function mutations in OsLAT5 confer resistance to methylviologen, providing a selectable marker for genome editing in rice.

Gibberellin Perception Sensors 1 and 2 reveal cellular GA dynamics articulated by COP1 and GA20ox1 that are necessary but not sufficient to pattern hypocotyl cell elongation

The paper uses a GA biosensor to show COP1 and GA20ox1 regulate GA gradients in hypocotyls, which are necessary but not sufficient for cell elongation during light and dark conditions.

Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei

The paper develops a non-invasive NIR imaging technique to visualize live plant cell nuclei using phytochrome-derived autofluorescence, applicable across multiple plant species.

A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes shift fluorescent protein

The paper introduces a new fluorescent protein, LSSmApple, and uses it to create a genetically encoded calcium sensor for yeast imaging.

Rice Yellow Mottle Virus resistance by genome editing of the Oryza sativa L. ssp. japonica nucleoporin gene OsCPR5.1 but not OsCPR5.2

This study uses CRISPR/Cas9 to edit OsCPR5.1 and OsCPR5.2 in rice to confer resistance to Rice Yellow Mottle Virus, finding that only OsCPR5.1 loss-of-function mutants are resistant.

Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains

The paper edits SWEET promoters in an African rice variety to confer resistance against Asian and African Xanthomonas oryzae pv. oryzae strains.

A high confidence Physcomitrium patens plasmodesmata proteome by iterative scoring and validation reveals diversification of cell wall proteins during evolution

The paper identifies a high-confidence plasmodesmata proteome in Physcomitrium patens using iterative scoring and validation, revealing evolutionary diversification of cell wall proteins.

SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14

The paper shows that SWEET13 transports sucrose, not gibberellin, is essential for male fertility in Arabidopsis, while GA transport is not required for this function.

OzTracs: Optical Osmolality Reporters Engineered from Mechanosensitive Ion Channels

This paper describes the engineering of osmolality sensors called OzTracs by fusing fluorescent proteins to mechanosensitive ion channels, showing they report osmolality changes through indirect mechanisms.

OsSWEET11b, a sixth leaf blight susceptibility gene involved in sugar transport-dependent male fertility

The paper identifies OsSWEET11b as a rice gene involved in male fertility and susceptibility to bacterial leaf blight, showing its role in sucrose transport and Xoo virulence.

Arabidopsis bZIP11 is a susceptibility factor during Pseudomonas syringae infection

This study identifies bZIP11 as a host transcription factor that promotes Pseudomonas syringae growth by regulating nutrient transporter expression in Arabidopsis.

Affinity series of genetically encoded high sensitivity Förster Resonance Energy Transfer sensors for sucrose

The paper develops a series of high-sensitivity Förster Resonance Energy Transfer sensors for sucrose, expanding detection range and enabling in vivo analysis of sucrose and trehalose.

Feedback inhibition of AMT1 NH4+-transporters mediated by CIPK15 kinase

This study identifies CIPK15 as a kinase that inhibits AMT1 ammonium transporters through phosphorylation, reducing NH4+ accumulation in plants.

Unique and distinct identities and functions of leaf phloem cells revealed by single cell transcriptomics

This study uses single-cell transcriptomics to identify and characterize distinct vascular cell types in Arabidopsis leaves, revealing unique functions of phloem parenchyma cells.

Phloem loading via the abaxial bundle sheath cells in maize leaves

The paper identifies abaxial bundle sheath cells in maize as a key site for phloem loading via SWEET and UmamiT transporters, revealing a unique mechanism distinct from Arabidopsis.

Sponging of glutamate at the outer plasma membrane surface reveals roles for glutamate in development

The paper shows that surface-displayed glutamate sensors affect plant development by sequestering glutamate at the plasma membrane, suggesting its role in signaling during growth and differentiation.

Self-labeling of proteins with chemical fluorescent dyes in BY-2 cells and Arabidopsis seedlings

The paper demonstrates effective self-labeling of proteins in plants using synthetic dyes for studying cell biological processes like endocytosis and microtubule dynamics.