A de novo designed enzyme for photo-proximity labeling of E3 ligase neighborhoods in live cells
Nam Hyeong Kim, Zhi Lin, Sangwon Nam, Danielle L. Swaney, Nevan J. Krogan, Yong Wook Kim, Michael J. Therien, William F. DeGrado, James A. Wells
This paper introduces EYClamp, a de novo designed enzyme for photo-proximity labeling of E3 ligase neighborhoods in live cells, identifying over 1,500 candidate neighbors for four E3 ligases.
bioRxiv · Jul 23, 2026 · PreprintBiochemistry