James A. Wells

This study shows that targeting LRP8 for degradation reduces GPX4 levels, sensitizing cancer cells to ferroptosis by disrupting selenium-dependent antioxidant defenses.

This study reveals how HSF1 is regulated by Hsp70 and Hsp90 chaperones through structural and mechanistic analyses, showing Hsp90's role in maintaining HSF1 in a pre-activated state.

This paper introduces EYClamp, a de novo designed enzyme for photo-proximity labeling of E3 ligase neighborhoods in live cells, identifying over 1,500 candidate neighbors for four E3 ligases.

The paper introduces contactMAP, a photocatalytic crosslinking method for high-resolution mapping of protein neighborhoods, showing it rivals or outperforms existing photo-PLP methods.

This paper expands structural surfaceomics to identify cancer-specific protein conformations across multiple tumor models, revealing potential immunotherapy targets through crosslinking mass spectrometry and structural modeling.

This paper develops Shedders, bispecific antibodies that recruit ADAM10 to induce ectodomain shedding of cell surface proteins, demonstrating efficacy in depleting LAG-3 and identifying new targets for therapeutic intervention.

The paper expands the kineTAC toolbox by creating 81 new cytokine receptor-targeting chimeras for cell-type-specific extracellular protein degradation.

This study identifies PAD4 substrates and their citrullination effects, showing PAD4 induces cell migration and generates citrullinated peptides that can trigger autoantibodies.

The paper uses spatiotemporal photocatalytic proximity labeling to track dynamic EGFR interactomes both extracellularly and intracellularly upon EGF activation.

This study shows that β-amyloid induces microglial GPC4 and APOE, which increases neuronal tau pathology and toxicity.

This paper develops LIPTACs, LDLR-targeting chimeras that enhance lysosomal delivery and cytotoxicity for cancer treatment by combining eTPD and ADC strategies.

This study identifies hypoxia-induced proteolytic remodeling of cell surface and secreted proteins in PDAC cell lines, highlighting potential biomarkers and therapeutic targets.

The paper uses CRISPRi to screen ASD risk genes in microglia, identifying ADNP's role in synaptic pruning and endocytosis.

This paper designs a protein to bind naphthalenediimides and evaluates its ability to generate reactive species for proximity labeling.

This study engineers a minimal EDV for genome editing by removing viral components, showing improved efficiency and reduced size.

The paper uses chemoproteomics to identify immunogenic cell surface substrates of CK2α in the tumor microenvironment, showing that extracellular phosphorylation can generate immunogenic antigens.

This study shows PVRL2 suppresses anti-tumor immunity independently of PVRIG and TIGIT, with deletion reducing tumor growth more than PD-L1 loss.

The paper identifies cell surface proteins upregulated in TSC1 mutant cells that offer therapeutic targets beyond mTORC1 inhibition.