A synthetic oligo library and sequencing approach reveals an insulation mechanism encoded within bacterial σ54 promoters
Lior Levy, Leon Anavy, Oz Solomon, Roni Cohen, Michal Brunwasser-Meirom, Shilo Ohayon, Orna Atar, Sarah Goldberg, Zohar Yakhini B, Roee Amit
The paper identifies an insulation mechanism in bacterial σ54 promoters using a synthetic oligo library and sequencing, showing CT-rich motifs reduce downstream gene expression.
Cell Reports · Oct 1, 2017Peer-reviewedSystems Biology