Roni Cohen

Transgene-Free Ex Utero Derivation of A Human Post-Implantation Embryo Model Solely from Genetically Unmodified Naive PSCs

This paper develops a transgene-free human post-implantation embryo model from genetically unmodified naive PSCs, recapitulating key developmental stages and extra-embryonic tissues.

Synthetic 5 UTRs can either up- or down-regulate expression upon RBP binding

The paper shows that synthetic 5 UTRs can be up- or down-regulated by RBP binding, revealing RNA-RBP interactions that control translation.

An in vivo binding assay for RNA-binding proteins based on repression of a reporter gene

The paper develops an in vivo method to quantify RNA-binding protein-RNA interactions using a reporter gene repression assay and SHAPE-seq in bacteria.

A synthetic oligo library and sequencing approach reveals an insulation mechanism encoded within bacterial σ54 promoters

The paper identifies an insulation mechanism in bacterial σ54 promoters using a synthetic oligo library and sequencing, showing CT-rich motifs reduce downstream gene expression.