Single-molecule nanopore-tweezers analysis of transcription elongation by intact and "stalk-less" yeast RNA polymerase II
Shuya Yang, Matthew Chen, Andrew H. Laszlo, Jonathan M. Craig, Jens H. Gundlach, Craig D. Kaplan, Richard H. Ebright
Nanopore tweezers enable monitoring of RNA polymerase II translocation relative to DNA during transcription elongation with single-nucleotide spatial resolution and millisecond-scale temporal resolution at biologically relevant, saturating substrate concentrations.
bioRxiv · Jul 22, 2026 · PreprintMolecular Biology