Richard H. Ebright

Nanopore tweezers enable monitoring of RNA polymerase II translocation relative to DNA during transcription elongation with single-nucleotide spatial resolution and millisecond-scale temporal resolution at biologically relevant, saturating substrate concentrations.

The paper reports structures of long-range coupled transcription-translation complexes (TTC-LC) in E. coli, showing how they differ from TTC-B and their roles in transcription-translation coupling.

This study characterizes the effects of three small-molecule inhibitors on MtbRNAP transcription elongation using optical tweezers, revealing distinct inhibition mechanisms and synergistic effects.

The paper reports the structure of the archaeal FttA pre-termination complex using cryo-EM, revealing its mechanism of action and functional homology with bacterial and eukaryotic systems.

Cryo-EM reveals the structural basis of transcription-translation coupling by RfaH.

The paper reports cryo-EM structures of transcription-translation complexes (TTCs) at different stages of the translation cycle in E. coli, revealing structural changes and their functional implications.

The paper reports cryo-EM structures of functional Rho pre-termination complexes in Escherichia coli, explaining their mechanism and resolving decades of research.

The paper identifies CTL0286 as the chlamydial RNA polymerase omega subunit and shows it is conserved across obligate intracellular bacteria.

This paper reports atomic structures of Qλ, showing it forms a nozzle to prevent RNA hairpin formation, unlike Q21, and uses a different mechanism and loading process involving NusA.

This study investigates reiterative transcription initiation, showing RNA extension occurs without DNA scrunching, involves a short RNA-DNA hybrid, and uses an alternative RNA exit pathway.

The paper uses cryo-EM to define structural states of transcription-translation complexes in bacteria that mediate NusG- and NusA-dependent coupling.

The paper uses single-molecule fluorescence resonance energy transfer to study RNA polymerase trigger loop dynamics, showing it closes and opens on the millisecond timescale and functions as a checkpoint for NTP identity and inhibitor target.

The paper reports high-resolution structures of Q protein states during antitermination, showing Q forms a nozzle that narrows the RNA-exit channel and prevents RNA hairpin formation.

This study reports crystal structures of ECF-sigma-factor-dependent transcription initiation complexes in Mycobacterium tuberculosis, revealing conserved interactions with RNAP and promoter DNA.

This study uses single-molecule imaging to show Escherichia coli RNA polymerase σ70 holoenzyme performs one-dimensional sliding on DNA during promoter search, even at high RNAP concentrations.

This paper reports the cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme bound to fidaxomicin, revealing its unique binding site and mechanism of transcription inhibition.

This study identifies the mechanism of transcription start site (TSS) selection by bacterial RNA polymerase (RNAP) through experimental assays showing DNA scrunching and antiscrunching.

This paper discovers pseudouridimycin, a novel antibacterial compound that inhibits bacterial RNA polymerase and shows potent activity against drug-resistant pathogens.

This study reports crystal structures of Mycobacterium tuberculosis RNA polymerase with and without antituberculosis drugs, revealing a new class of inhibitors and their binding mechanisms.